PDT treatment in FL + PDT was less painful, significantly during the second treatment (P = 0.026). Little pain was perceived in most patients during PDT illumination. RESULTS: An excellent efficacy of 92.9% (sBCC, 100% BD, 80%) was found in both CL + PDT and FL + PDT after 12 months. Patients were also asked for their preferred treatment. An equivalence analysis was performed on the primary endpoint efficacy, while secondary endpoints pain, side-effects and aesthetics were evaluated using paired samples tests. ![]() This treatment was repeated after 2 weeks. Laser treatment was followed by MAL application and illumination occurred 3 h later. METHODS: Thirty treatment areas in fifteen patients with inoperable, histologically verified sBCC or BD received CL or FL after intrapatient randomization. OBJECTIVE: To compare a continuous (CL) and a fractional (FL) ablative CO2 laser-assisted methyl aminolevulinate (MAL) PDT in the management of superficial basal cell carcinoma (sBCC) and Bowen's disease (BD). Furthermore, xenogen-free isolation and autologous plasma-augmented culture expansion of ASCs, combined with the existing clinical experience with Glyaderm®, aid in simplifying the necessary procedures in a GMP-laboratory setting.īACKGROUND: Laser-assisted photodynamic therapy is being explored as a method to enhance efficacy of photodynamic therapy (PDT). The results of this study encourage clinical trials for treatment of full-thickness skin defects. ASC-seeded Glyaderm® significantly enhances wound healing compared to standard Glyaderm®. CONCLUSION: Glyaderm® is an effective carrier to deliver ASCs in full-thickness wounds. EGFP-ASCs could be retrieved by immunohistochemistry in the granulation tissue in early wound healing, and lining vascular structures in later stages. Granulation thickness and vascularization were significantly increased during early wound healing. Re-epithelialization in the ASC-seeded Glyaderm® side was significantly increased, resulting in complete wound healing in 12 days. EGFP-tranduction resulted in 95% positive cells after sorting. ![]() RESULTS: ASCs were characterized with flowcytometric analysis and differentiation assay. Hypoxic culture of the ASCs was performed to evaluate cytokine production. EGFP-staining allowed for tracking of the ASCs in vivo. Statistical analysis was conducted using the Wilcoxon signed-rank test. Re-epithelialization rate, granulation thickness and vascularity were assessed by histology on days 3, 7 and 12. Glyaderm® discs were seeded with EGFP-transduced ASCs, and implanted on 8â¯mm full-thickness dorsal wounds in an immunodeficient murine model, in comparison to standard Glyaderm® discs. METHODS: Adipose tissue was obtained from abdominal liposuction, and stromal cells were isolated mechanically and cultured xenogen-free in autologous plasma-supplemented medium. ![]() In this study, we investigate the compatibility of xenogen-free isolated human ASCs seeded on human acellular dermal matrix (Glyaderm®) in a murine immunodeficient wound model. Adipose-derived stem cells (ASCs) have been described to enhance wound healing through structural and humoral mechanisms. Dermal substitutes are currently used together with skin grafts in a single treatment to reconstruct the dermal layer of the skin, resulting in improved quality of scars. OBJECTIVE: Full-thickness cutaneous wounds treated with split-thickness skin grafts often result in unaesthetic and hypertrophic scars.
0 Comments
Leave a Reply. |